Small-molecule bioanalytical sample preparation method development starting from the BASICS

Abstract

Aim: To develop a robust small-molecule bioanalytical sample preparation method, systematic investigations of the bioanalytical handling conditions of analytes and IS of interest are preferred. So far, such investigations are done manually and are labor-intensive and error-prone. Result: An automation-assisted system has been developed to facilitate such systematic investigations. The system takes experimental design and automates the majority of the wet laboratory work. In addition, the system also automates the data extraction, recovery/loss computation, graphing and reporting. Conclusion: The automation-assisted system greatly reduces errors and labor involved in the systematic investigation of analytes and IS both for wet chemistry experiments and for data extraction and processing, enhances data processing efficiency and overall sample preparation method development productivity.