A disposable sampling device to collect volume-measured DBS directly from a fingerprick onto DBS paper-Reference-Cited by-同舟云学术

A disposable sampling device to collect volume-measured DBS directly from a fingerprick onto DBS paper

Published:2015-09 Issue:16 Volume:7 Page:2085-2094
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Lenk Gabriel¹,Sandkvist Sören²,Pohanka Anton²,Stemme Göran¹,Beck Olof²,Roxhed Niclas¹

Affiliation:

1. Department for Micro & Nanosystems, KTH Royal Institute of Technology, Osquldas väg 10, 10044 Stockholm, Sweden

2. Department of Clinical Pharmacology, Karolinska University Hospital, 14152 Stockholm, Sweden

Abstract

Background: DBS samples collected from a fingerprick typically vary in volume and homogeneity and hence make an accurate quantitative analysis of DBS samples difficult. Results: We report a prototype which first defines a precise liquid volume and subsequently stores it to a conventional DBS matrix. Liquid volumes of 2.2 µl ± 7.1% (n = 21) for deionized water and 6.1 µl ± 8.8% (n = 15) for whole blood have been successfully metered and stored in DBS paper. Conclusion: The new method of collecting a defined volume of blood by DBS sampling has the potential to reduce assay bias for the quantitative evaluation of DBS samples while maintaining the simplicity of conventional DBS sampling.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.15.134

Reference18 articles.

1. Evaluation of a dried blood spot HIV-1 RNA program for early infant diagnosis and viral load monitoring at rural and remote healthcare facilities

2. Dried Blood Spot Methods in Therapeutic Drug Monitoring: Methods, Assays, and Pitfalls

3. Quantitative Analysis of Therapeutic Drugs in Dried Blood Spot Samples by Paper Spray Mass Spectrometry: An Avenue to Therapeutic Drug Monitoring

4. Application of dried blood spots combined with HPLC-MS/MS for the quantification of acetaminophen in toxicokinetic studies

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