Evaluation of two blood microsampling approaches for drug discovery PK studies in rats-Reference-Cited by-同舟云学术

Evaluation of two blood microsampling approaches for drug discovery PK studies in rats

Published:2015-09 Issue:18 Volume:7 Page:2345-2359
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Luo Yongyi¹,Korfmacher Walter¹,Ho Stacy¹,Shen Liduo¹,Wang Jie¹,Wu Zhongtao¹,Guo Yang¹,Snow Gregory²,O'Shea Thomas¹

Affiliation:

1. Drug Metabolism & Pharmacokinetics, Sanofi, Second Avenue, Waltham, MA 02451, USA

2. Agilux, 1 Innovation Drive, Worcester, MA 01605, USA

Abstract

Background: Serial sampling in discovery rat PK studies could be performed via capillary microsampling (CMS) of blood or by using the Mitra™ device to collect dried blood samples. Results: Blood CMS results were compared with Mitra sampling results for four test compounds dosed in rat PK studies. The PK profiles obtained from CMS sampling were found to be very similar to those obtained from the Mitra sampling. For 15-µl blood CMS samples, freezing before the dilution step was found to be acceptable. Conclusion: Blood CMS using 15-µl glass capillary microsamples works well for serial blood sampling in rat PK studies. The Mitra microsampling device provides an alternative method for collecting 10 µl of blood as a dried blood sample.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.15.126

Reference20 articles.

1. Foundation review: Principles and applications of LC-MS in new drug discovery

2. HPLC-API/MS/MS: a powerful tool for integrating drug metabolism into the drug discovery process

3. Validation of a bioanalytical method using capillary microsampling of 8 µl plasma samples: application to a toxicokinetic study in mice

4. Capillary microsampling of 25 µl blood for the determination of toxicokinetic parameters in regulatory studies in animals

5. Capillary microsampling in the regulatory environment: validation and use of bioanalytical capillary microsampling methods

Cited by 26 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. LC-MS/MS measurement of endogenous steroid hormones and phase II metabolites in blood volumetric absorptive microsampling (VAMS) for doping control purposes;Clinica Chimica Acta;2024-04

2. Analytical and Clinical Validation of Assays for Volumetric Absorptive Microsampling (VAMS) of Drugs in Different Blood Matrices: A Literature Review;Molecules;2023-08-14

3. Validation and application of volumetric absorptive microsampling (VAMS) dried blood method for phenylalanine measurement in patients with phenylketonuria;Clinical Biochemistry;2023-06

4. Applications of Volumetric Absorptive Microsampling Technique: A Systematic Critical Review;Therapeutic Drug Monitoring;2023-03-06

5. Volumetric absorptive microsampling–LC–MS/MS assays for quantitation of giredestrant in dried human whole blood;Bioanalysis;2022-11