Molecular diversity of rice (Oryza sativa L.) genotypes in Malaysia based on SSR markers

Author:

ANISUZZAMAN Mohammad,ISLAM Mohammad Rafiqul,KHATUN Hasina,HAQUE Mohammad Amdadul,ISLAM Mahammad Shariful,AHSAN Mohammad Shamim

Abstract

Rice crop improvement is determined by the degree of genetic variability and the heritability of favorable genes. A total of twenty-five SSR markers were used to measure the level of polymorphism and genetic variation among the 65 rice genotypes. Twenty-one of the twenty-five SSRs were discovered to be polymorphic, whereas the rest were determined to be monomorphic. A total of 91 alleles were found in 21 SSR markers, with an average of 4.00 alleles which ranged from 3 (RM335, RM551, RM538 RM190, RM242 and RM270) to 7 (RM263). The average PIC value was 0.62 ranging from 0.28 (RM 270) to 0.76 (RM 481). The rice genotypes were divided into nine primary clusters by a dendrogram based on NTSYS software’s UPGMA analysis. The cluster analysis revealed that these genotypes were divided into nine clusters where cluster IB-1a has the most genotypes (31) followed by cluster IB-1b (24).The genotype BR24 and Utri as well as Pukhi and WANGI PUTEH had the highest dissimilarity coefficient values indicating genotype diversity. These accessions have a lot of genetic diversity among the constituents; thus, they could be used directly in a hybridization program to improve yield-related parameters.

Publisher

University of Ljubljana

Subject

General Agricultural and Biological Sciences,Water Science and Technology

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