Affiliation:
1. State Scientific Center of Dermatology, Venerology and Cosmetology (Moscow, Russia)
Abstract
Objective.
To investigate prevalence of the A2058G mutation in the Russian population of T. pallidum and its association with molecular subtypes.
Materials and Methods.
We analyzed DNA isolated from 325 samples of clinical material obtained from patients of dermatovenereological treatment and prophylactic institutions in 6 federal districts of the Russia in the period from 2014 to 2021. Patients were diagnosed with primary syphilis of the genital organs, primary syphilis of other sites, or secondary syphilis of the skin and mucous membranes. DNA was isolated using the Proba-NK reagent kit (DNA-technology, Russia) according to the manufacturer’s instructions. The presence of T. pallidum genetic material was confirmed by PCR with primers for the species-specific polA gene. Molecular typing was performed based on the analysis of polymorphic regions of species-specific T. pallidum genes. The primary decoding of the nucleotide sequences of the 23S rRNA gene fragment was carried out using the Sequencing Analysis 5.3.1 program. The analyzed fragments were aligned using the Mega 5 program.
Results.
Eight molecular subtypes of T. pallidum – 14d/f, 14d/g, 14b/f, 14c/f, 14i/f, 9d/f, 14b/g and 14e/f with stable dominance of subtype 14d/f – were identified in the Russian Federation in the interval 2014–2021. Three subtypes, 14d/g, 14b/g and 14b/f, carrying the A2058G mutation associated with azithromycin resistance, were identified.
Conclusions.
Studies on molecular typing of T. pallidum strains in the Russia have shown significant population heterogeneity. Three sublines containing the A2058G mutation were shown to exist, one of which – 14b/f – is described as rare. The obtained data confirm the relevance of continuous monitoring of the emergence of resistant strains and the development of new mutations.
Publisher
Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy
Subject
Pharmacology (medical),Infectious Diseases,Microbiology (medical),Epidemiology
Reference32 articles.
1. World Health Organization. The global health observatory. Data on syphilis. Available at: www.who.int/data/gho/data/themes/topics/topic-details/GHO/data-on-syphilis. Accessed July 21, 2021.
2. World Health Organization. New study highlights unacceptably high global prevalence of syphilis among men who have sex with men. Available at: www.who.int/news/item/09-07-2021-new-study-highlightsunacceptably-high-global-prevalence-of-syphilis-amongmen-who-have-sex-with-men. Accessed July 9, 2021.
3. Kubanov A.A., Bogdanova E.V. Dermatovenereology of Russian Federation in 2020: working under pandemic. Vestnik dermatologii i venerologii. 2021;97(4):8-32. Russian. DOI: 10.25208/vdv1261
4. Kotova E.G., Kobyakova O.S., Kubanov A.A., Starodubov V.I., Aleksandrova G.A., Bogdanova E.V., et al. Resources and activities of medical organizations of the dermatovenerological profile. The incidence of sexually transmitted infections, contagious skin diseases and skin diseases in 2021: statistical materials. M.: Russian Research Institute of Health of the Ministry of Health of Russia, 2022; 209 p. Russian.
5. Centers for Disease Control and Prevention. Brief report: azithromycin treatment failures in syphilis infections – San Francisco, California, 2002-2003. MMWR Morb Wkly Rep. 2004;53:197-198. PMID: 15017376.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献