DYNAMICS OF PRETREATED WHEAT STRAW SACCHARIFICATION BY CELLULOSOME OF Trichoderma viride

Abstract

The main goal of this study was to analyze the potential of Trichoderma viride BEOFB 1210m for cellulase production during solid-state fermentation of wheat straw pretreated with the ligninosome of a well-known white-rot delignificator – Pleurotus pulmonarius HAI 573. After only 7 days of T. viridae BEOFB 1210m cultivation on biologically pretreated wheat straw, this micromycete produced the most active xylanases, which were also the dominant enzymes, with a value of even 3730.10 U L-1. Likewise, maximal but much lower values of exocellulases (155.83 U L-1) and β-glucosidases (59.98 U L-1) were detected after the same period of fermentation. However, much higher activity of endocellulase (2439.55 U L-1) was obtained on the 10th day. The dynamics of enzyme activity was reflected on the level of substrate depolymerization. As much as 30.56% of the cellulose was degraded already on the 7th day, and that percentage did not change significantly until the end of the cultivation period. A significant loss of hemicelluloses was also measured at the beginning of the fermentation process, but it reached a maximum of approximately 50% by the 21st day. The results clearly showed that the selected T. viride strain has very good potential to synthesize highly active cellulases when grown on a cheap and available substrate, which is significant for further large-scale industrial applications.