Abstract
The aim of the present study was to evaluate the hepatoprotective effect of total saponins and tannins of Dialium guineense stem bark in Wistar rats exposed to carbon tetrachloride (CCl4). Adult male Wistar rats (n = 25) weighing 160 – 180 g (mean weight = 170 ± 10 g) were randomly assigned to five groups (5 rats per group): normal control, CCl4 control, silymarin, total saponins, and total tannins groups. With the exception of normal control, the rats were exposed to CCl4 (a single oral dose of 1.0 mL/kg body weight, bwt). Rats in the silymarin group were administered 100 mg/kg bwt silymarin (standard hepatoprotective drug), while those in the two treatment groups received 150 mg/kg bwt of total saponins or tannins orally. Treatment lasted 28 days. Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and Alkaline Phosphatase (ALP), as well as levels of Total Protein (TP), bilirubin, and albumin, were measured in plasma. The results showed that there were no significant differences in the concentrations of TP among the groups (p > 0.05). The activities of the liver enzymes, as well as levels of bilirubin and albumin, were significantly higher in the CCl4 control group than in the normal control group, but they were reduced by extract treatment (p < 0.05). These results indicate that CCl4 negatively impacted the integrity of the liver cells, and total saponins and tannins of D. guineense stem bark conferred some level of protection on the organ.
Publisher
Peertechz Publications Private Limited
Reference26 articles.
1. 1. Gupta M, Mazumder UK, Das S. Effect of alkaloidal extract from Clerodendron colebrookianum on hematological parameters and hepatorenal functions in mice. Indian J Exp Biol. 1994 Mar;32(3):189-91. PMID: 8070840.
2. 2. Friedman SE, Grendel JH, McQuaid KR. Current Diagnosis and Treatment in Gastroenterology. New York. Lange Medical Books, McGraw Hill. 2003; 664-679.
3. 3. Junnila M, Rahko T, Sukura A, Lindberg LA. Reduction of carbon tetrachloride-induced hepatotoxic effects by oral administration of betaine in male Han-Wistar rats: a morphometric histological study. Vet Pathol. 2000 May;37(3):231-8. doi: 10.1354/vp.37-3-231. PMID: 10810987.
4. 4. Cui CP, Wei P, Liu Y, Zhang DJ, Wang LS, Wu CT. The protective role of Hepatopoietin Cn on liver injury induced by carbon tetrachloride in rats*. Hepatol Res. 2009 Feb;39(2):200-6. doi: 10.1111/j.1872-034X.2008.00447.x. Epub 2008 Nov 26. PMID: 19054144.
5. 5. Kim HY, Kim JK, Choi JH, Jung JY, Oh WY, Kim DC, Lee HS, Kim YS, Kang SS, Lee SH, Lee SM. Hepatoprotective effect of pinoresinol on carbon tetrachloride-induced hepatic damage in mice. J Pharmacol Sci. 2010;112(1):105-112. doi: 10.1254/jphs.09234fp. PMID: 20093790.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献