Research progress of near-infrared fluorescence immunoassay

Abstract

Near-infrared fl uorescence immunoassay has been widely studied in the bio analytical fi eld. This review mainly introduces the basic principles of near-infrared spectroscopy and near-infrared detection technology, and summarizes the properties, characteristics and recent improvement of optical properties and signal intensity of three near-infrared fl uorescence probes (i.e. organic fl uorophores, quantum dots and rare earth compounds). We describe the applications of near-infrared fl uorescence technology in immunoassay, and prospect the application potential of lateral fl ow assay (LFA) based on this probe in the rapid detection of pathogens. Our team intends to establish a new platform which has highly sensitive near-infrared fl uorescence probes (NIFPs) combined with portable and simple immunochromatographic test strips (ICTSs) for rapid detection of food borne viruses. This will provide technical support for a rapid detection on the port.