HPA-3 and C807T polymorphisms are associated with laboratory biomarkers of coronary artery disease in Brazilian women

Abstract

Reports describe the association between platelet activation, adhesion, and aggregation, as well as polymorphisms in genes encoding platelet membrane glycoproteins with a predisposition to coronary artery disease (CAD). This study investigates associations between HPA-1, -3 and C807T polymorphisms and CAD. A cross-sectional study involving 114 women who underwent coronary angiography was performed. The CAD+ group consisted of 63 women while the CAD- group of 51 women. Hematological and biochemical parameters were evaluated by electronic methods. Polymorphisms were investigated by PCR-RFLP and PCR-SSP and participants answered an epidemiological questionnaire and provided anthropometric data. Blood pressure was also measured. Statistical analyses were performed using EpiInfo v. 7.0 software. Results showed that age, diabetes mellitus, hypertension, and hyperlipidemia were associated with CAD. In addition, CAD+ women showed elevated glucose, TG, and VLDL-c levels and reduced HDL-c levels. No significant difference was observed between the allelic and genotypic distributions of the polymorphisms HPA-1, -3, and C807T in CAD+ and CAD- groups. However, CAD+ women carriers of the variant 3b allele had elevated TG and sP-selectin levels while the carriers of the variant 807T allele had reduced CT and LDL-c levels. Hence, our data suggest that the polymorphism HPA-3 increases, indirectly, the risk of CAD development and occurrence while polymorphism C807T reduces this effec.