Scientific Justification of the Selection of the Optimum Composition of the Phytocomposition Included in Phytosomal Gel for the Treatment of Cuperosis

Abstract

Purpose: The research work aims to carry out the acquisition of phytoextracts from green tea leaves, hop cones, and horse chestnut seeds with the participation of various extraction methods for the future preparation of phytosomal gel in the treatment of couperosis, to study their technological and physico-chemical properties. Methods: In the scientific research laboratory of the Department of Pharmaceutical Technology of the Azerbaijan Medical University, a phytocomposition was developed in 5 variants from green tea leaves, hop cones, and horse chestnut seeds in different proportions. Phytoextract was obtained from compositions prepared by maceration and high-speed extraction methods. 70% ethyl alcohol was used as an extractant in the research work. The extraction process was performed twice for each composition. Results: Researches were performed with a high-speed extraction method (High-Speed Dispersator- STEGLER DG-360, 26-28 °C, 50Hs, 2800-28000 cycles/min). The extraction time was 7 minutes in the 1st stage, 3 minutes in the 2nd stage, temperature 26 °C, frequency 50 Hz, power 360 W, 3300 cycles/min. Extracts were also obtained by the maceration method in a cone-shaped glass macerator for 3 days. Physico-chemical properties of extracts (organoleptic properties, dry residue, density, pH, amount of heavy metals) were studied. According to the results of the research, the amount of extractive substances obtained in the Stegler DG 360 model device - a high-speed homogenizer was higher. During the comparative studies conducted with phytocompositions, compositions No. 3 and 5 showed more different results according to the relevant criteria.