Efficacy of Iodophor to Inactivate <i>Aeromonas hydrophila</i> Biofilm on Two Subtrates

Abstract

The multiple day old biofilms of Aeromonas hydrophila on two substrates, high density polyethylene (HDPE) plastic and Eichhornia crassipes leaves were evaluated for its susceptibility to bactericidal action of iodophor at two dose levels (20 mg/l and 40 mg/l). The biofilm on HDPE was highly resistant to the killing action of iodophor on the days 2, 4 and 6 tested at 20 and 40 mg/l for 10 min. The iodophor was effective in inactivating the biofilm cells on Eichhornia at 40 mg/l for 10 min, whereas at 20 mg/l for 10 min, the 4 and 6 days old biofilm on Eichhornia showed resistance to iodophor. The planktonic cells were completely killed at 20 mg/l of iodophor. The study indicates that biofilms of A. hydrophila were resistant to iodophor in comparison to planktonic cells.