Sub-lethal Ammonia Toxicity in Fingerlings of <i>Catla catla</i> (ham.) and <i>Labeo rohita</i> (ham.): Effect on Certain Haematological Parameters and Enzymes Activities

Author:

Das P. C.,Ayyappan S.,Jena J. K.

Abstract

Fingerlings of catla and rohu were exposed to ambient sub-lethal total ammonia-nitrogen concentrations of 1, 2, 4, 8, 11.8 and 16 mg/l for 96 hours to study the toxic effect. Haematological parameters such as TEC and TLC increased in both species in lower concentration of ambient ammonia but decreased in higher concentration while haemoglobin and serum protein content decreased and blood glucose level increased with increasing ammonia concentration. Elevation of LDH, ALAT and ASAT activities were observed in serum and different tissues while inhibition was recorded in AChE activity in brain and liver with increasing ammonia concentration. Comparison of the haematological responses of the two major carps revealed catla to exhibit significant alteration in almost all haematological parameters at relatively lower ammonia concentrations compared to that of rohu. The study on enzymes also revealed lower alteration in AChE, LDH, ALAT and ASAT activities in rohu suggesting better tolerance of the species to higher ambient ammonia than catla, corroborating catla to be more susceptible to environmental stress than rohu.

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