The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack

Author:

Tie Hieng Chiong1ORCID,Ludwig Alexander1ORCID,Sandin Sara12,Lu Lei1ORCID

Affiliation:

1. School of Biological Sciences, Nanyang Technological University, Singapore, Singapore

2. NTU Institute of Structural Biology, Nanyang Technological University, Singapore, Singapore

Abstract

It is unclear how the two principal functions of the Golgi complex, processing and transport, are spatially organized. Studying such spatial organization by optical imaging is challenging, partially due to the dense packing of stochastically oriented Golgi stacks. Using super-resolution microscopy and markers such as Giantin, we developed a method to identify en face and side views of individual nocodazole-induced Golgi mini-stacks. Our imaging uncovered that Golgi enzymes preferentially localize to the cisternal interior, appearing as a central disk or inner-ring, whereas components of the trafficking machinery reside at the periphery of the stack, including the cisternal rim. Interestingly, conventional secretory cargos appeared at the cisternal interior during their intra-Golgi trafficking and transiently localized to the cisternal rim before exiting the Golgi. In contrast, bulky cargos were found only at the rim. Our study therefore directly demonstrates the spatial separation of processing and transport functions within the Golgi complex.

Funder

Ministry of Education - Singapore

National Medical Research Council

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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