Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P2 in Drosophila

Author:

Lattner Johanna1ORCID,Leng Weihua1,Knust Elisabeth1ORCID,Brankatschk Marko2ORCID,Flores-Benitez David1ORCID

Affiliation:

1. Max-Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany

2. The Biotechnological Center of the TU Dresden (BIOTEC), Dresden, Germany

Abstract

An efficient vectorial intracellular transport machinery depends on a well-established apico-basal polarity and is a prerequisite for the function of secretory epithelia. Despite extensive knowledge on individual trafficking pathways, little is known about the mechanisms coordinating their temporal and spatial regulation. Here, we report that the polarity protein Crumbs is essential for apical plasma membrane phospholipid-homeostasis and efficient apical secretion. Through recruiting βHeavy-Spectrin and MyosinV to the apical membrane, Crumbs maintains the Rab6-, Rab11- and Rab30-dependent trafficking and regulates the lipid phosphatases Pten and Ocrl. Crumbs knock-down results in increased apical levels of PI(4,5)P2 and formation of a novel, Moesin- and PI(4,5)P2-enriched apical membrane sac containing microvilli-like structures. Our results identify Crumbs as an essential hub required to maintain the organization of the apical membrane and the physiological activity of the larval salivary gland.

Funder

Max-Planck-Gesellschaft

Deutsche Forschungsgemeinschaft

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Reference213 articles.

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