Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome-Reference-Cited by-同舟云学术

Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome

Published:2016-01-26 Issue: Volume:5 Page:
ISSN:2050-084X
Container-title:eLife
language:en
Short-container-title:

Author:

Fang Qianglin¹²,Chen Ping¹,Wang Mingzhu¹,Fang Junnan¹²,Yang Na¹²,Li Guohong¹²,Xu Rui-Ming¹²^ORCID

Affiliation:

1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

Abstract

Human cytomegalovirus (hCMV) immediate early 1 (IE1) protein associates with condensed chromatin of the host cell during mitosis. We have determined the structure of the chromatin-tethering domain (CTD) of IE1 bound to the nucleosome core particle, and discovered that the specific interaction between IE1-CTD and the H2A-H2B acidic patch impairs the compaction of higher-order chromatin structure. Our results suggest that IE1 loosens up the folding of host chromatin during hCMV infections.

Funder

Ministry of Science and Technology

National Natural Science Foundation of China

Chinese Academy of Sciences

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Link

https://cdn.elifesciences.org/articles/11911/elife-11911-v3.pdf

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