NINscope, a versatile miniscope for multi-region circuit investigations

Author:

de Groot Andres1,van den Boom Bastijn JG12ORCID,van Genderen Romano M34,Coppens Joris1,van Veldhuijzen John1,Bos Joop1,Hoedemaker Hugo1,Negrello Mario34ORCID,Willuhn Ingo12ORCID,De Zeeuw Chris I14ORCID,Hoogland Tycho M14ORCID

Affiliation:

1. Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands

2. Department of Psychiatry, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands

3. Faculty of Applied Sciences, TU Delft, Delft, Netherlands

4. Department of Neuroscience, Erasmus MC, Rotterdam, Netherlands

Abstract

Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform acquisition and control software, our miniscope is a versatile addition to the expanding tool chest of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.

Funder

Koninklijke Nederlandse Akademie van Wetenschappen

H2020 European Research Council

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Topsector Life Sciences & Health

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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