Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division

Author:

Gray Andrew N1,Egan Alexander JF2,van't Veer Inge L3,Verheul Jolanda4,Colavin Alexandre5,Koumoutsi Alexandra6ORCID,Biboy Jacob2,Altelaar A F Maarten7,Damen Mirjam J7,Huang Kerwyn Casey589,Simorre Jean-Pierre10,Breukink Eefjan3,den Blaauwen Tanneke4,Typas Athanasios6,Gross Carol A11112,Vollmer Waldemar2

Affiliation:

1. Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, United States

2. Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle Upon Tyne, United Kingdom

3. Membrane Biochemistry and Biophysics, Bijvoet Centre for Biomolecular Research, University of Utrecht, Utrecht, The Netherlands

4. Bacterial Cell Biology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Amsterdam, The Netherlands

5. Biophysics Program, Stanford University, Stanford, United States

6. Genome Biology Unit, European Molecular Biology Laboratory Heidelberg, Heidelberg, Germany

7. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht, The Netherlands

8. Department of Bioengineering, Stanford University, Stanford, United States

9. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, United States

10. Institut de Biologie Structurale, Université Grenoble Alpes, Grenoble, France

11. Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, United States

12. California Institute of Quantitative Biology, University of California, San Francisco, San Francisco, United States

Abstract

To maintain cellular structure and integrity during division, Gram-negative bacteria must carefully coordinate constriction of a tripartite cell envelope of inner membrane, peptidoglycan (PG), and outer membrane (OM). It has remained enigmatic how this is accomplished. Here, we show that envelope machines facilitating septal PG synthesis (PBP1B-LpoB complex) and OM constriction (Tol system) are physically and functionally coordinated via YbgF, renamed CpoB (Coordinator of PG synthesis and OM constriction, associated with PBP1B). CpoB localizes to the septum concurrent with PBP1B-LpoB and Tol at the onset of constriction, interacts with both complexes, and regulates PBP1B activity in response to Tol energy state. This coordination links PG synthesis with OM invagination and imparts a unique mode of bifunctional PG synthase regulation by selectively modulating PBP1B cross-linking activity. Coordination of the PBP1B and Tol machines by CpoB contributes to effective PBP1B function in vivo and maintenance of cell envelope integrity during division.

Funder

Wellcome Trust

National Institutes of Health (NIH)

Alexander von Humboldt-Stiftung

European Molecular Biology Laboratory

European Commission

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

National Science Foundation (NSF)

Stanford University

Netherlands Organisation for Scientific Research

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Cited by 156 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3