Immune surveillance of the lung by migrating tissue monocytes

Author:

Rodero Mathieu P123,Poupel Lucie123,Loyher Pierre-Louis123,Hamon Pauline123,Licata Fabrice123,Pessel Charlotte123,Hume David A4,Combadière Christophe123,Boissonnas Alexandre123

Affiliation:

1. Centre d'Immunologie et des Maladies Infectieuses, University Pierre et Marie Curie, Sorbonne Universities, Paris, France

2. Centre d'Immunologie et des Maladies Infectieuses, Institut national de la santé et de la recherche médicale, U1135, Paris, France

3. Centre d'Immunologie et des Maladies Infectieuses, Centre national de la recherche scientifique, ERL 8255, Paris, France

4. Royal (Dick) School of Veterinary Studies, The Roslin Institute, Midlothian, United Kingdom

Abstract

Monocytes are phagocytic effector cells in the blood and precursors of resident and inflammatory tissue macrophages. The aim of the current study was to analyse and compare their contribution to innate immune surveillance of the lung in the steady state with macrophage and dendritic cells (DC). ECFP and EGFP transgenic reporters based upon Csf1r and Cx3cr1 distinguish monocytes from resident mononuclear phagocytes. We used these transgenes to study the migratory properties of monocytes and macrophages by functional imaging on explanted lungs. Migratory monocytes were found to be either patrolling within large vessels of the lung or locating at the interface between lung capillaries and alveoli. This spatial organisation gives to monocytes the property to capture fluorescent particles derived from both vascular and airway routes. We conclude that monocytes participate in steady-state surveillance of the lung, in a way that is complementary to resident macrophages and DC, without differentiating into macrophages.

Funder

European Commission (EC)

Ligue Contre le Cancer

Association pour la Recherche sur le Cancer

Agence Nationale de la Recherche

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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