Shugoshin biases chromosomes for biorientation through condensin recruitment to the pericentromere

Author:

Verzijlbergen Kitty F1,Nerusheva Olga O1,Kelly David1,Kerr Alastair1,Clift Dean1,de Lima Alves Flavia1,Rappsilber Juri12,Marston Adele L1

Affiliation:

1. Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh, United Kingdom

2. Department of Biotechnology, Institute of Bioanalytics, Technische Universität Berlin, Berlin, Germany

Abstract

To protect against aneuploidy, chromosomes must attach to microtubules from opposite poles (‘biorientation’) prior to their segregation during mitosis. Biorientation relies on the correction of erroneous attachments by the aurora B kinase, which destabilizes kinetochore-microtubule attachments that lack tension. Incorrect attachments are also avoided because sister kinetochores are intrinsically biased towards capture by microtubules from opposite poles. Here, we show that shugoshin acts as a pericentromeric adaptor that plays dual roles in biorientation in budding yeast. Shugoshin maintains the aurora B kinase at kinetochores that lack tension, thereby engaging the error correction machinery. Shugoshin also recruits the chromosome-organizing complex, condensin, to the pericentromere. Pericentromeric condensin biases sister kinetochores towards capture by microtubules from opposite poles. Our findings uncover the molecular basis of the bias to sister kinetochore capture and expose shugoshin as a pericentromeric hub controlling chromosome biorientation.

Funder

Wellcome Trust

EMBO

Scottish Universities Life Sciences Alliance

Darwin Trust of Edinburgh

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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