Meisosomes, folded membrane microdomains between the apical extracellular matrix and epidermis

Author:

Aggad Dina1ORCID,Brouilly Nicolas2ORCID,Omi Shizue1ORCID,Essmann Clara Luise34ORCID,Dehapiot Benoit2ORCID,Savage-Dunn Cathy5ORCID,Richard Fabrice2ORCID,Cazevieille Chantal6,Politi Kristin A7ORCID,Hall David H7ORCID,Pujol Remy6ORCID,Pujol Nathalie1ORCID

Affiliation:

1. Aix Marseille Univ, INSERM, CNRS, CIML, Turing Centre for Living Systems

2. Aix Marseille Université, CNRS, IBDM, Turing Centre for Living Systems

3. Department of Computer Science, University College London

4. Bio3/Bioinformatics and Molecular Genetics, Albert-Ludwigs-University

5. Department of Biology, Queens College and the Graduate Center, CUNY

6. INM, Institut des Neurosciences de Montpellier, Plateau de microscopie électronique, INSERM, Université de Montpellier

7. Department of Neuroscience, Albert Einstein College of Medicine

Abstract

Apical extracellular matrices (aECMs) form a physical barrier to the environment. In Caenorhabditis elegans, the epidermal aECM, the cuticle, is composed mainly of different types of collagen, associated in circumferential ridges separated by furrows. Here, we show that in mutants lacking furrows, the normal intimate connection between the epidermis and the cuticle is lost, specifically at the lateral epidermis, where, in contrast to the dorsal and ventral epidermis, there are no hemidesmosomes. At the ultrastructural level, there is a profound alteration of structures that we term ‘meisosomes,’ in reference to eisosomes in yeast. We show that meisosomes are composed of stacked parallel folds of the epidermal plasma membrane, alternately filled with cuticle. We propose that just as hemidesmosomes connect the dorsal and ventral epidermis, above the muscles, to the cuticle, meisosomes connect the lateral epidermis to it. Moreover, furrow mutants present marked modifications of the biomechanical properties of their skin and exhibit a constitutive damage response in the epidermis. As meisosomes co-localise to macrodomains enriched in phosphatidylinositol (4,5) bisphosphate, they could conceivably act, like eisosomes, as signalling platforms, to relay tensile information from the aECM to the underlying epidermis, as part of an integrated stress response to damage.

Funder

Agence Nationale de la Recherche

National Institutes of Health

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Cited by 4 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3