Reevaluation of Piezo1 as a gut RNA sensor

Author:

Nickolls Alec R1ORCID,O'Brien Gabrielle S1,Shnayder Sarah1,Zhang Yunxiao2,Nagel Maximilian1,Patapoutian Ardem2ORCID,Chesler Alexander T1ORCID

Affiliation:

1. National Center for Complementary and Integrative Health, National Institutes of Health

2. Department of Neuroscience, Howard Hughes Medical Institute, Dorris Neuroscience Center, Scripps Research Institute

Abstract

Piezo1 is a stretch-gated ion channel required for mechanosensation in many organ systems. Recent findings point to a new role for Piezo1 in the gut, suggesting that it is a sensor of microbial single-stranded RNA (ssRNA) rather than mechanical force. If true, this would redefine the scope of Piezo biology. Here, we sought to replicate the central finding that fecal ssRNA is a natural agonist of Piezo1. While we observe that fecal extracts and ssRNA can stimulate calcium influx in certain cell lines, this response is independent of Piezo1. Additionally, sterilized dietary extracts devoid of gut biome RNA show similar cell line-specific stimulatory activity to fecal extracts. Together, our data highlight potential confounds inherent to gut-derived extracts, exclude Piezo1 as a receptor for ssRNA in the gut, and support a dedicated role for Piezo channels in mechanosensing.

Funder

National Center for Complementary and Integrative Health

National Institute of Neurological Disorders and Stroke

National Center for Advancing Translational Sciences

Howard Hughes Medical Institute

National Institutes of Health

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Piezo1 in Digestive System Function and Dysfunction;International Journal of Molecular Sciences;2023-08-19

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