Targeting of the Fun30 nucleosome remodeller by the Dpb11 scaffold facilitates cell cycle-regulated DNA end resection

Author:

Bantele Susanne CS1,Ferreira Pedro2,Gritenaite Dalia1,Boos Dominik2,Pfander Boris1ORCID

Affiliation:

1. DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Martinsried, Germany

2. Centre for Medical Biotechnology, Molecular Genetics II, University Duisburg-Essen, Essen, Germany

Abstract

DNA double strand breaks (DSBs) can be repaired by either recombination-based or direct ligation-based mechanisms. Pathway choice is made at the level of DNA end resection, a nucleolytic processing step, which primes DSBs for repair by recombination. Resection is thus under cell cycle control, but additionally regulated by chromatin and nucleosome remodellers. Here, we show that both layers of control converge in the regulation of resection by the evolutionarily conserved Fun30/SMARCAD1 remodeller. Budding yeast Fun30 and human SMARCAD1 are cell cycle-regulated by interaction with the DSB-localized scaffold protein Dpb11/TOPBP1, respectively. In yeast, this protein assembly additionally comprises the 9-1-1 damage sensor, is involved in localizing Fun30 to damaged chromatin, and thus is required for efficient long-range resection of DSBs. Notably, artificial targeting of Fun30 to DSBs is sufficient to bypass the cell cycle regulation of long-range resection, indicating that chromatin remodelling during resection is underlying DSB repair pathway choice.

Funder

Deutsche Forschungsgemeinschaft

Max-Planck-Gesellschaft

Fonds der Chemischen Industrie

NRW Rueckkehrerprogramm from the state of North-Rhine-Westphalia

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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