Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast

Author:

Odermatt Pascal D12ORCID,Miettinen Teemu P34ORCID,Lemière Joël1ORCID,Kang Joon Ho356ORCID,Bostan Emrah7,Manalis Scott R389,Huang Kerwyn Casey21011ORCID,Chang Fred1ORCID

Affiliation:

1. Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, United States

2. Department of Bioengineering, Stanford University, Stanford, United States

3. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, United States

4. MRC Laboratory for Molecular Cell Biology, University College, London, United Kingdom

5. Department of Physics, Massachusetts Institute of Technology, Cambridge, United States

6. Brain Science Institute, Korea Institute of Science and Technology, Seoul, Republic of Korea

7. Informatics Institute, University of Amsterdam, Amsterdamn, Netherlands

8. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, United States

9. Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, United States

10. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, United States

11. Chan Zuckerberg Biohub, San Francisco, United States

Abstract

Intracellular density impacts the physical nature of the cytoplasm and can globally affect cellular processes, yet density regulation remains poorly understood. Here, using a new quantitative phase imaging method, we determined that dry-mass density in fission yeast is maintained in a narrow distribution and exhibits homeostatic behavior. However, density varied during the cell cycle, decreasing during G2, increasing in mitosis and cytokinesis, and dropping rapidly at cell birth. These density variations were explained by a constant rate of biomass synthesis, coupled to slowdown of volume growth during cell division and rapid expansion post-cytokinesis. Arrest at specific cell-cycle stages exacerbated density changes. Spatially heterogeneous patterns of density suggested links between density regulation, tip growth, and intracellular osmotic pressure. Our results demonstrate that systematic density variations during the cell cycle are predominantly due to modulation of volume expansion, and reveal functional consequences of density gradients and cell-cycle arrests.

Funder

Swiss National Science Foundation

National Institute of General Medical Sciences

Wellcome Trust

Chan Zuckerberg Initiative

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Cited by 40 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3