Zygotic pioneer factor activity of Odd-paired/Zic is necessary for late function of the Drosophila segmentation network

Author:

Soluri Isabella V1,Zumerling Lauren M1,Payan Parra Omar A23ORCID,Clark Eleanor G2,Blythe Shelby A1ORCID

Affiliation:

1. Department of Molecular Biosciences, Northwestern University, Evanston, United States

2. Program in Interdisciplinary Biological Sciences, Northwestern University, Evanston, United States

3. Department of Neurobiology, Northwestern University, Evanston, United States

Abstract

Because chromatin determines whether information encoded in DNA is accessible to transcription factors, dynamic chromatin states in development may constrain how gene regulatory networks impart embryonic pattern. To determine the interplay between chromatin states and regulatory network function, we performed ATAC-seq on Drosophila embryos during the establishment of the segmentation network, comparing wild-type and mutant embryos in which all graded maternal patterning inputs are eliminated. While during the period between zygotic genome activation and gastrulation many regions maintain stable accessibility, cis-regulatory modules (CRMs) within the network undergo extensive patterning-dependent changes in accessibility. A component of the network, Odd-paired (opa), is necessary for pioneering accessibility of late segmentation network CRMs. opa-driven changes in accessibility are accompanied by equivalent changes in gene expression. Interfering with the timing of opa activity impacts the proper patterning of expression. These results indicate that dynamic systems for chromatin regulation directly impact the reading of embryonic patterning information.

Funder

Searle Leadership Fund for Life Sciences

Northwestern University

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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