Affiliation:
1. MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University
2. State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University
Abstract
Autophagy-related gene 6 (ATG6) plays a crucial role in plant immunity. Nonexpressor of pathogenesis-related genes1 (NPR1) acts as a signaling hub of plant immunity. However, the relationship between ATG6 and NPR1 is unclear. Here, we find that ATG6 directly interacts with NPR1.
ATG6
overexpression significantly increased nuclear accumulation of NPR1. Furthermore, we demonstrate that
ATG6
increases NPR1 protein levels and improves its stability. Interestingly, ATG6 promotes the formation of SINCs (SA-induced NPR1 condensates)-like condensates. Additionally, ATG6 and NPR1 synergistically promote the expression of
pathogenesis-related
genes. Further results showed that silencing
ATG6
in
NPR1-GFP
exacerbates
Pst
DC3000/
avrRps4
invasion, while double overexpression of
ATG6
and
NPR1
synergistically inhibits
Pst
DC3000/
avrRps4
invasion. In summary, our findings unveil an interplay of NPR1 with ATG6 and elucidate important molecular mechanisms for enhancing plant immunity.We unveil a novel relationship in which ATG6 positively regulates NPR1 in plant immunity.
Publisher
eLife Sciences Publications, Ltd
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