The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

Author:

Khalifa Ahmad Abdelzaher Zaki12ORCID,Ichikawa Muneyoshi1ORCID,Dai Daniel12ORCID,Kubo Shintaroh23ORCID,Black Corbin Steven12ORCID,Peri Katya1ORCID,McAlear Thomas S12ORCID,Veyron Simon24ORCID,Yang Shun Kai12ORCID,Vargas Javier12ORCID,Bechstedt Susanne12ORCID,Trempe Jean-François24ORCID,Bui Khanh Huy12ORCID

Affiliation:

1. Department of Anatomy and Cell Biology, McGill University, Québec, Canada

2. Centre de Recherche en Biologie Structurale - FRQS, McGill University, Québec, Canada

3. Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto, Japan

4. Department of Pharmacology & Therapeutics, McGill University, Montréal, Canada

Abstract

Microtubules are cytoskeletal structures involved in stability, transport and organization in the cell. The building blocks, the α- and β-tubulin heterodimers, form protofilaments that associate laterally into the hollow microtubule. Microtubule also exists as highly stable doublet microtubules in the cilia where stability is needed for ciliary beating and function. The doublet microtubule maintains its stability through interactions at its inner and outer junctions where its A- and B-tubules meet. Here, using cryo-electron microscopy, bioinformatics and mass spectrometry of the doublets of Chlamydomonas reinhardtii and Tetrahymena thermophila, we identified two new inner junction proteins, FAP276 and FAP106, and an inner junction-associated protein, FAP126, thus presenting the complete answer to the inner junction identity and localization. Our structural study of the doublets shows that the inner junction serves as an interaction hub that involves tubulin post-translational modifications. These interactions contribute to the stability of the doublet and hence, normal ciliary motility.

Funder

Canadian Institutes of Health Research

Natural Sciences and Engineering Research Council of Canada

Canadian Institute for Advanced Research

Japan Society for the Promotion of Science

Abdulla Al Ghurair Foundation for Education

Canada Research Chairs

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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