Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

Author:

Serra-Marques Andrea1ORCID,Martin Maud1ORCID,Katrukha Eugene A1,Grigoriev Ilya1,Peeters Cathelijn AE1,Liu Qingyang1,Hooikaas Peter Jan1ORCID,Yao Yao2,Solianova Veronika1,Smal Ihor2,Pedersen Lotte B3ORCID,Meijering Erik2ORCID,Kapitein Lukas C1ORCID,Akhmanova Anna1ORCID

Affiliation:

1. Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands

2. Departments of Medical Informatics and Radiology, Biomedical Imaging Group Rotterdam, Erasmus University Medical Center, Rotterdam, Netherlands

3. Department of Biology, Section of Cell Biology and Physiology, the August Krogh Building, University of Copenhagen, Copenhagen, Denmark

Abstract

Intracellular transport relies on multiple kinesins, but it is poorly understood which kinesins are present on particular cargos, what their contributions are and whether they act simultaneously on the same cargo. Here, we show that Rab6-positive secretory vesicles are transported from the Golgi apparatus to the cell periphery by kinesin-1 KIF5B and kinesin-3 KIF13B, which determine the location of secretion events. KIF5B plays a dominant role, whereas KIF13B helps Rab6 vesicles to reach freshly polymerized microtubule ends, to which KIF5B binds poorly, likely because its cofactors, MAP7-family proteins, are slow in populating these ends. Sub-pixel localization demonstrated that during microtubule plus-end directed transport, both kinesins localize to the vesicle front and can be engaged on the same vesicle. When vesicles reverse direction, KIF13B relocates to the middle of the vesicle, while KIF5B shifts to the back, suggesting that KIF5B but not KIF13B undergoes a tug-of-war with a minus-end directed motor.

Funder

H2020 European Research Council

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

H2020 Marie Skłodowska-Curie Actions

Fundação para a Ciência e a Tecnologia

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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