A conserved cell-pole determinant organizes proper polar flagellum formation

Author:

Arroyo-Pérez Erick Eligio12,Hook John C.3,Alvarado Alejandra4,Wimmi Stephan15,Glatter Timo6,Thormann Kai M.3,Ringgaard Simon12

Affiliation:

1. Max Planck Institute for Terrestrial Microbiology, Department of Ecophysiology

2. Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München

3. Department of Microbiology and Molecular Biology, Justus-Liebig-Universität Gießen

4. Interfaculty Institute of Microbiology and Infection Medicine Tübingen, Bacterial Metabolomics, University of Tübingen

5. Institute for Biological Physics, University of Cologne

6. Core Facility for Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology

Abstract

The coordination of cell cycle progression and flagellar synthesis is a complex process in motile bacteria. In γ-proteobacteria, the localization of the flagellum to the cell pole is mediated by the SRP-type GTPase FlhF. However, the mechanism of action of FlhF, and its relationship with the cell pole landmark protein HubP remain unclear. In this study, we discovered a novel protein called FipA that is required for normal FlhF activity and function in polar flagellar synthesis. We demonstrated that membrane-localized FipA interacts with FlhF and is required for normal flagellar synthesis in Vibrio parahaemolyticus , Pseudomonas putida , and Shewanella putrefaciens , and it does so independently of the polar localization mediated by HubP. FipA exhibits a dynamic localization pattern and is present at the designated pole before flagellar synthesis begins, suggesting its role in licensing flagellar formation. This discovery provides insight into a new pathway for regulating flagellum synthesis and coordinating cellular organization in bacteria that rely on polar flagellation and FlhF-dependent localization.

Publisher

eLife Sciences Publications, Ltd

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