Transcriptomic profiling of tissue environments critical for post-embryonic patterning and morphogenesis of zebrafish skin
Author:
Aman Andrew J1ORCID,
Saunders Lauren M2ORCID,
Carr August A1,
Srivatasan Sanjay2,
Eberhard Colten3ORCID,
Carrington Blake3,
Watkins-Chow Dawn3,
Pavan William J3,
Trapnell Cole2,
Parichy David M14ORCID
Affiliation:
1. Department of Biology, University of Virginia
2. Department of Genome Sciences, University of Washington
3. National Human Genome Research Institute, National Institutes of Health
4. Department of Cell Biology, University of Virginia
Abstract
Pigment patterns and skin appendages are prominent features of vertebrate skin. In zebrafish, regularly patterned pigment stripes and an array of calcified scales form simultaneously in the skin during post-embryonic development. Understanding the mechanisms that regulate stripe patterning and scale morphogenesis may lead to the discovery of fundamental mechanisms that govern the development of animal form. To learn about cell types and signaling interactions that govern skin patterning and morphogenesis, we generated and analyzed single-cell transcriptomes of skin from wild-type fish as well as fish having genetic or transgenically induced defects in squamation or pigmentation. These data reveal a previously undescribed population of epidermal cells that express transcripts encoding enamel matrix proteins, suggest hormonal control of epithelial–mesenchymal signaling, clarify the signaling network that governs scale papillae development, and identify a critical role for the hypodermis in supporting pigment cell development. Additionally, these comprehensive single-cell transcriptomic data representing skin phenotypes of biomedical relevance should provide a useful resource for accelerating the discovery of mechanisms that govern skin development and homeostasis.
Funder
National Institute of General Medical Sciences
National Institute of Arthritis and Musculoskeletal and Skin Diseases
National Heart, Lung, and Blood Institute
National Human Genome Research Institute
Paul G. Allen Frontiers Group
Publisher
eLife Sciences Publications, Ltd
Subject
General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience
Cited by
1 articles.
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