Local and global influences on protein turnover in neurons and glia

Author:

Dörrbaum Aline R12ORCID,Kochen Lisa1,Langer Julian D13ORCID,Schuman Erin M1ORCID

Affiliation:

1. Max Planck Institute for Brain Research, Frankfurt, Germany

2. Faculty of Biological Sciences, Goethe University Frankfurt, Frankfurt, Germany

3. Max Planck Institute of Biophysics, Frankfurt, Germany

Abstract

Regulation of protein turnover allows cells to react to their environment and maintain homeostasis. Proteins can show different turnover rates in different tissue, but little is known about protein turnover in different brain cell types. We used dynamic SILAC to determine half-lives of over 5100 proteins in rat primary hippocampal cultures as well as in neuron-enriched and glia-enriched cultures ranging from <1 to >20 days. In contrast to synaptic proteins, membrane proteins were relatively shorter-lived and mitochondrial proteins were longer-lived compared to the population. Half-lives also correlate with protein functions and the dynamics of the complexes they are incorporated in. Proteins in glia possessed shorter half-lives than the same proteins in neurons. The presence of glia sped up or slowed down the turnover of neuronal proteins. Our results demonstrate that both the cell-type of origin as well as the nature of the extracellular environment have potent influences on protein turnover.

Funder

Horizon 2020 Framework Programme

Max-Planck-Gesellschaft

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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