Affiliation:
1. Department of Quality Assurance, P.R. Pote Patil College of Pharmacy, Amravati – 444604.
2. Department of Pharmaceutics, P.R. Pote Patil College of Pharmacy, Amravati – 444604.
Abstract
A rapid, sensitive and accurate method for Estimation of Anti- Histamine in Tablet Dosage Form by RP-HPLC. Mobile phase was Methanol: 0.1%. Ortho phosphoric acid (80:20% v/v). Flow rate was 0.9ml/min. The detection Wavelength was 227nm. The injection volume was 20µl.The proposed method was validated for selectivity, precision, linearity, and accuracy. All validation parameters were within the acceptable range. Linearity studies for Rupatadine Fumarate were performed (10-60µg/ml). The %RSD for accuracy found to be less than 2%. Assay was found to be 99.17 to 101.65 respectively. The range of 10-60µg /ml was selected for the linearity of a standard Rupatadine Fumarate. The 80%, 100% and 120% levels of recovery study were selected to perform the recovery study. Linearity studies for Rupatadine Fumarate was performed on (10-60µg/ml).
Reference18 articles.
1. Malviya VR, Pande SD, Bobade NN. Preparation and Evaluation of Sustained Release Beads of Zolmitriptan Hydrochloride. Research Journal of Pharmacy and Technology. 2019;12(12):5972-6.
2. Shaiba M, Devi K, Prashanthi R, Raghavi K, Sindhura M. High Performance Thin Layer Chromatographic Estimation of Rupatadine Fumerate. Research Journal of Pharmacy and Technology. 2011;4(3):420-2.
3. Fidan V, Fidan T. Fixed drug eruption against rupatadine fumarate. Journal of Craniofacial Surgery. 2011 Sep 1;22(5):1682-3.
4. Malviya VR, Pande SD. Road CKN. Preparation ad Evaluation of Zolmitriptan Hydrochloride Lozenge. J Pharma Res. 2019;8(8):624-9.
5. Pulla RP, Sastry BS, Prasad YR, Raju NA. Simultaneous Estimation of Metformin HCl and Sitagliptin Phosphate in Tablet Dosage Forms by RP-HPLC. Research Journal of Pharmacy and Technology. 2011;4(4):646-9.