In Vitro Comparative Study on the Antibacterial and the Antioxidant Activity of Pergularia tomentosa L.

Abstract

Ethanolic extracts of Pergularia tomentosa L. were used for the relative analysis of antioxidant and antimicrobial activity against two types of Gram-negative bacteria. (Escherichia coli G (-) and Pseudomonas aeruginosa G (-)) and a gram positive (Staphylococus aureus G (+)). Maximum activity was recorded against Escherichia coli, with a maximum inhibition diameter of 23mm using the disk diffusion method. In addition, the results revealed a higher bacterial activity against Escherichia coli with an inhibition diameter of 23mm compared to standard antibiotics Ampicillin/Nystatin with an inhibition diameter of 11.75mm. The study of the antioxidant activity by the DPPH and FRAP methods showed that vitamin C is the most active with an IC50 equal to 0.010 g/ml, followed by the crude extract, with a value of 0.012 g/ml and finally Tocopherol (VE) which seems to be the least effective with an IC50 of 0.015 g/ml. The results obtained in the present study suggest that Pergularia tomentosa L. can be used in the treatment of diseases caused by the organisms tested. Further chemical and pharmacological investigations can be carried out to isolate and identify the chemical constituents in selected plants responsible for antimicrobial activity.