The impact of pH value in the Phenolic content and Antioxidant potential of Medicinal plant extract-Reference-Cited by-同舟云学术

The impact of pH value in the Phenolic content and Antioxidant potential of Medicinal plant extract

Published:2022-10-01 Issue: Volume: Page:331-336
ISSN:0974-4150
Container-title:Asian Journal of Research in Chemistry
language:en
Short-container-title:AJRC

Author:

Djamila Berra¹,Ouahida Dia²,Salah Eddine Laouini¹,Souheila Menaceur³

Affiliation:

1. Department of Process Engineering, Faculty of Technology, University of Echahid Hamma Lakhdar, El Oued 39000, Algeria.

2. Unit of Renewable Energy Development in Arid Zones (UDERZA), El Oued 39000, Algeria.

3. Laboratoire De Biotechnologie Biomatériaux et Matériaux Condensés, Faculty of Technology, University Echahid Hammam Lakhdar El Oued Algeria.

Abstract

In this research, the aim was to investigate the influence of pH value in the extraction medium on the phytochemical composition and antioxidant activity of Artemisia herba-alba species used in traditional medicine. For this reason, two analytical methods were used (UV-visible spectroscopy, HPLC). Antioxidant properties of the extracts at different pH were estimated by two tests: total antioxidant capacity (TAC) and DPPH free radical scavenging capacity. The results of the evaluation of phenolic compounds showed that the aqueous extracts in a weak acid medium had a high percentage of phenol content, and the highest value for total phenol content was 90.759±1.267mg GAE/g. Whereas, the HPLC analysis indicated the presence of most of the standard phenolic compounds in the majority of the extracts. For antioxidant activity, the best antioxidants reported in extract at pH=6, with a semi-maximal inhibitory concentration (0.151±0,032mg/ml) and a total antioxidant capacity (TAC) of 66.840±0.241mg EAA/g, which indicates that it is effective antioxidant for therapeutic applications.

Publisher

A and V Publications

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