The impact of pH value in the Phenolic content and Antioxidant potential of Medicinal plant extract-Reference-Cited by-同舟云学术

The impact of pH value in the Phenolic content and Antioxidant potential of Medicinal plant extract

Published:2022-10-01 Issue: Volume: Page:331-336
ISSN:0974-4150
Container-title:Asian Journal of Research in Chemistry
language:en
Short-container-title:AJRC

Author:

Djamila Berra¹,Ouahida Dia²,Salah Eddine Laouini¹,Souheila Menaceur³

Affiliation:

1. Department of Process Engineering, Faculty of Technology, University of Echahid Hamma Lakhdar, El Oued 39000, Algeria.

2. Unit of Renewable Energy Development in Arid Zones (UDERZA), El Oued 39000, Algeria.

3. Laboratoire De Biotechnologie Biomatériaux et Matériaux Condensés, Faculty of Technology, University Echahid Hammam Lakhdar El Oued Algeria.

Abstract

In this research, the aim was to investigate the influence of pH value in the extraction medium on the phytochemical composition and antioxidant activity of Artemisia herba-alba species used in traditional medicine. For this reason, two analytical methods were used (UV-visible spectroscopy, HPLC). Antioxidant properties of the extracts at different pH were estimated by two tests: total antioxidant capacity (TAC) and DPPH free radical scavenging capacity. The results of the evaluation of phenolic compounds showed that the aqueous extracts in a weak acid medium had a high percentage of phenol content, and the highest value for total phenol content was 90.759±1.267mg GAE/g. Whereas, the HPLC analysis indicated the presence of most of the standard phenolic compounds in the majority of the extracts. For antioxidant activity, the best antioxidants reported in extract at pH=6, with a semi-maximal inhibitory concentration (0.151±0,032mg/ml) and a total antioxidant capacity (TAC) of 66.840±0.241mg EAA/g, which indicates that it is effective antioxidant for therapeutic applications.

Publisher

A and V Publications

Reference41 articles.

1. Gurib-Fakim A. Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular aspects of Medicine. 2006; 27(1):1-93. https://doi.org/10.1016/j.mam.2005.07.008.

2. Killedar S G. More H N. Screening of antimicrobial potential and phytoconsituents for different extracts of Memecylon umbellatum Burm inflorescences. Asian Journal of Pharmaceutical Research. 2011; 1(4):114-118.

3. Muthul C. Muniappan A. Nagappan R. Savarimuthu I. Medicinal plants used by traditional healers in Kancheepuram District of Tamil Nadu. J Ethnobiol Ethnomed. 2006; 2(43):43. https://doi.org/10.1186/1746-4269-2-43.

4. Elayarani M. Shanmuganathan P. Muthukumaran P. In vitro anti-oxidant activity of the various extracts of Cassia auriculata L. flower by UV spectrophotometer. Asian Journal of Pharmacy and Technology. 2011; 1(3):70-72.

5. Karmakar I . Dolai N. Saha P. Sarkar N . Bala A. Haldar P K. Scavenging activity of Curcuma caesia rhizome against reactive oxygen and nitrogen species. Oriental Pharmacy and Experimental Medicine. 2011; 11(4):221-228, 20. https://doi.org/10.1007/s13596-011-0030-6.

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Antioxidant activity test of Macerated extract and Sonicated extract of Areca nut (Areca catechu L.);Research Journal of Pharmacy and Technology;2023-12-29

2. The growth of ETV6-NTRK3 harbouring cells was inhibited by Artemisia vulgaris L. crude extract;Research Journal of Pharmacy and Technology;2023-08-31