Development and Validation of UV Spectroscopic Method for content Analysis of Sparfloxacin using Hydrotropic Solubilization

Abstract

This present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost-effective UV spectroscopic method for the analysis of Sparfloxacin in bulk and pharmaceutical formulation using hydrotropic solubilization. The solvent used for this work was 20% sodium benzoate solution as hydrotropic solubilizing agent. The quantitative determination of the drug was carried out at 292nm and Beer’s law was obeyed in the range of 2-20μg/mL. The method was shown the linearity of above concentrations with regression equation y = 0.5186x – 0.0132 with correlation coefficient of R² = 0.9992. The proposed method was statistically validated for accuracy, repeatability and ruggedness as per ICH guidelines. The percentage content for precision studies and percentage recoveries determined for Sparfloxacin marketed tablet formulations were close to 100 with low %RSD. Hence, the proposed method was accurate, cost effective and enough precise.