Simultaneous determination of Sulphadoxine and Pyrimethamine by taking Tolterodine as an internal standard in Pharmaceutical dosage form by RP-HPLC-Reference-Cited by-同舟云学术

Simultaneous determination of Sulphadoxine and Pyrimethamine by taking Tolterodine as an internal standard in Pharmaceutical dosage form by RP-HPLC

Published:2021-05-13 Issue: Volume: Page:145-150
ISSN:2231-5675
Container-title:Asian Journal of Pharmaceutical Analysis
language:en
Short-container-title:AJPA

Author:

Patil Sushil D.¹,Shelke Pravin B.²,Aher Priti²,Hafizur Rahman Maswood Ahmed²

Affiliation:

1. Department of Pharmaceutical Chemistry, MET's Institute of Pharmacy, Bhujbal Knowledge City, Adgaon Nashik, Maharashtra, India.

2. MET’s Institute of Pharmacy, MET League of Colleges, Bhujbal Knowledge City, Adgaon, Nashik, Maharashtra State 422003, India.

Abstract

A simple, rapid, economic, sensitive and precise HPLC method has been developed for the simultaneous determination of Sulphadoxine and Pyrimethamine in pharmaceutical dosage form by taking Tolterodine as an internal standard. The method was carried out using Phenomenex C18 (4.6ID × 250mm; 5µm) column and mobile phase comprised of methanol and Phosphate Buffer in proportion of ratio 60:40 v/v. The flow rate was 1.0mL/min and detection was carried out at 276nm. The retention time of Sulphadoxine, Pyrimethamine and Tolterodine were found to be 2.967, 4.058 and 6.908 respectively. Linearity of Sulphadoxine and Pyrimethamine in the range of 2 to 12μg/mL and 4 to 24μg/mL respectively. The % recoveries of Sulphadoxine and Pyrimethamine were found to be in between 99.93% to 99. 96 % respectively. The proposed method is suitable for the routine quality control analysis for simultaneous determination of Sulphadoxine and Pyrimethamine was in bulk and pharmaceutical dosage form.

Publisher

A and V Publications

Reference9 articles.

1. Christen GD. Analytical Chemistry. 5th ed. John Wiley and Sons; 2003; 35-42, 131-132.

2. Sharma BK. Instrumental Methods of Chemical Analysis. 25th ed. Meerut: Goel Publication Co; 1983; 3: 6.

3. Skoog DA, Holler FJ, Crouch SR. Principle of Instrumental Analysis. 6th ed. India: Thomson Publications; 2007; 1-3: 145-147, 180.

4. ICH, Q2 (R1); Validation of analytical procedures: text and methodology. International Conference on Harmonization, Geneva; 2005; 1- 13.

5. Nash R.; Pharmaceutical Process Validation; 3rd edition; Marcel Dekker Publication; New York; 107; 2002: 340-370.