Analytical Method Development and Validation for Simultaneous estimation of Methotrexate and Hydroxychloroquine sulfate in bulk drug by using RP-HPLC-Reference-Cited by-同舟云学术

Analytical Method Development and Validation for Simultaneous estimation of Methotrexate and Hydroxychloroquine sulfate in bulk drug by using RP-HPLC

Published:2021-05-13 Issue: Volume: Page:73-78
ISSN:2231-5675
Container-title:Asian Journal of Pharmaceutical Analysis
language:en
Short-container-title:AJPA

Author:

Sharma Anupam¹,Ankalgi Amar Deep¹,Devi Arti¹,Pandit Vinay¹,Singh Ashawat Mahendra¹

Affiliation:

1. Department of Pharmaceutical Analysis and Quality Assurance, Laureate Institute of Pharmacy, VPO Kathog, Jawalamukhi, Himachal Pradesh - 176031, India

Abstract

A rapid isocratic chromatographic procedure for the analysis of methotrexate, hydroxychloroquine sulfate in bulk drug and pharmaceutical formulations was developed validated in the present study. The mobile phase consists of a mixture of Water: Acetonitrile: Tetrahydrofuran in the proportion of 50:40:10 and pH maintained to 3with perchloric acid. Retention time was found to be 3.0 and 3.7minutes for methotrexate and hydroxychloroquine sulfate respectively. The method was performing by using the C18 column, ODS Hypersil column with UV detection at 318nm, and flow rate of 1.0ml/min. The percentage of recovery for both drugs was found to be 99.99%. All validation parameters were within limits as per the ICH guidelines.

Publisher

A and V Publications

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