Affiliation:
1. B. K. Mody Government Pharmacy College, Polytechnic Campus, Near Aji Dam, Rajkot, Gujarat, India.
2. Government Pharmacy College, Gandhinagar, Gujarat, India.
Abstract
High performance liquid chromatography (HPLC) is an analytical technique which is adept to separate, detect and quantify numerous pharmaceuticals and their related degradants. It is also used to identify and measure synthetic drugs, to minimise other impurities at the time of separation, and to separate manufactured medicines from drug-related contaminants. To improve the procedure, a number of chromatographic parameters were assessed. It is necessary to identify the best mobile phase, column, detector, buffer, wavelength, and gradient for the drug's compatibility and stability with degradants and contaminants. This study discusses the significance of RP-HPLC in the development of analytical methods, their techniques, and a brief overview of the key chromatographic parameters that must be tuned for effective method development.
Reference33 articles.
1. Settle FA. In: Handbook of Instrumental Techniques for Analytical Chemistry. 1st Edn; Pearson Education Inc, Singapore.2004.
2. Durai T, Kumar A, Sai Charan. Evolution of liquid chromatography: Technologies and applications. International Journal of Research in Pharmaceutical Sciences. 2020; 11(3): 3204-3211.
3. Snyder LR, Kirkland JJ, Glach JL. Practical HPLC Method Development. John Wiley and Sons. New York. 1997; pp. 158-192.
4. Shethi PD. HPLC-Quantitative analysis of pharmaceutical formulations. CBS Publishers & Distributors. 1997; 3rdEdn: pp. 182-184.
5. Yadav V, Bharkatiya M.A Review on HPLC Method Development and Validation. Research journal of life sciences, Bioinformatics, Pharmaceuticals and Chemical Sciences. 2017; 2(6): 166-178.