Development and Validation of RP-HPLC Method for the Estimation of Linezolid from Bulk and Formulations-Reference-Cited by-同舟云学术

Development and Validation of RP-HPLC Method for the Estimation of Linezolid from Bulk and Formulations

Published:2023-11-23 Issue: Volume: Page:273-277
ISSN:2231-5675
Container-title:Asian Journal of Pharmaceutical Analysis
language:en
Short-container-title:AJPA

Author:

Farkade Kalyani¹,Salode Vikrant²,Kadu Tejashri³

Affiliation:

1. P.R. Pote Patil College of Pharmacy, Amaravati, MS, India.

2. P.R. Patil Institute of Pharmacy, Amaravati, MS, India.

3. Institute of Pharmacy and Research, Badnera, MS, India.

Abstract

A new, simple, rapid, selective, precise and accurate isocratic reverse-phase high-performance liquid Chromatography assay method has been developed to estimate Linezolid in tablet formulations. The separation was achieved using column Inert sustain C18 (250×4.6mm, 5µ) in the mobile phase consisting of methanol: 1% Acetic acid (50:50 v/v). The flow rate was 0.80mL/min-1, and the separated Linezolid was detected using a UV detector at 249nm. Column temperature 45°C and sample temperature ambient and injection volume 20µL. The retention time of Linezolid was noted to be 7.3min. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent.

Publisher

A and V Publications

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