Estimation of Andrographolide Content in Aqueous and Methanolic Extracts of Marketed Nilavembu Kudineer Siddha formulations by HPTLC Technique

Abstract

Andrographis paniculata is one of the important ingredient used in various Siddha formulations. The present study is mainly aimed to compare various marketed nilavembukudineer churna on the basis of andrographolide content. HPTLC method was used for this determination using marker compound andrographolide. The HPTLC method was performed using HPTLC aluminium sheets precoated with silica gel 60 GF254 as stationary phase and ethyl acetate: n-hexane (8.5:1.5 v/v) as the mobile phase. The developed chromatogram was scanned at 233nm using Camag scanner III. The reference standard andrographolide Rf value was found to be 0.55.Fromthe five market formulations screened, the andrographolide content in methanolic and aqueous extracts showed the discrepancy in content amount representing by order MFM-1 MFA-1 MFM-2 MFA-2 -3.The other market formulation extracts both methanol and aqueous extracts does not confirms the presence of andrographolide.