Preliminary Phytochemical Screening and Study of In vitro Antibacterial Activity on H37RV Strain using roots of Ziziphus nummularia

Abstract

Objective: To evaluate the invitro antimicrobial and anti-tuberculosis activity by screening its bacterial resistance using Ziziphus nummularia root extracts. Methods: Ziziphus nummularia root extracts such as aqueous and ethanol were prepared by using soxhalation process. The antimicrobial activity was screened by agar well diffusion method and antituberculosis activity were screened by MABA (Microplate Alamar Blue Assay). Results and conclusion: In preliminary phytochemical screening the extracts showed the presence of major phytoconstituents such as alkaloid, flavonoid, tannin, saponin, terpenoid, sterols, carbohydrate and protein. The Minimum inhibitory concentration (MIC) were noted in both invitro activity screening. In Antimicrobial screening, the ethanolic extract of Ziziphus nummularia root was more susceptible to Staphylococci with a maximum zone of inhibition 24mm, while aqueous extract showed zone of inhibition 14mm at 80 mg/ml. It shows that ethanolic extract of Ziziphus nummularia root has good potential antimicrobial activity against aqueous extract and standard drug. Similarly in antituberculosis activity, the aqueous extract of Ziziphus nummularia root showed minimum inhibitory at a concentration of 25 μg/ml, while ethanolic extract showed minimum inhibitory concentration at 100 μg/ml. The aqueous extract of Ziziphus nummularia has moderate antituberculosis activity when compared with ethanolic extract and standard drug we used.