Evaluation of TRICHONEMTM as Anti-Dandruff and Hair growth promoter in young adults

Abstract

The aim of the present study is to formulate and evaluate herbal Anti dandruff hair wash powder containing eighteen herbal ingredients and soluble egg shell membrane in a proportionate combination with an emphasis on safety and efficacy. It contains scientific blends of Poduthalai (or) Turkey tangle frog fruit, Shikakai, Pulankelangu (or) White turmeric, Amla extarct, Curry leaf powder, Venthayam (or) Fenugreek, Pasipayiru (or) Mung bean, Vettiveru (or) Vetivergrass, Rose powder, Sembaruti (or) Hibiscus powder, Avaram (or) Tanner's cassia, Kadukkai (or) Indian hog plum, Lady’s finger powder, Redcabsol (or) Red cabbage powder, Arappu leaf powder, Tulsi extract, Neam Leaf powder, Karisalankanni (or) False daisy powder and Soluble egg shell Membrane. With respect to the prior art data each ingredient has several independent studies to prove that they have Anti-bacterial, Anti-fungal, Anti-dandruff and Hair growth promoting properties. Soluble Egg Shell Membrane (Enzyme) used in the preparation of TRICHONEMTM consists of different types of collagens (I, III, V, X), glycosaminoglycan, hyaluronic acid, keratin and elastin. In this study all the selected ingredients were sieved using mesh, weighed accurately and mixed geometrically for uniform formulation and then evaluated for parameters including Organoleptic, physicochemical, physical, irritancy along with stability examination. The dried powder of combined form had passable flow property which is suitable for a hair wash powder. The pH of all formulations observed are near to neutral range. During irritancy test, TRICHONEMTM hair wash powder formulation does not express any type of irritation on skin and eyes. Hence the results proved that hair wash powder formulations are suitable for its intended use. In vitro Antidandruff Activity of TRICHONEM TM hair wash powder against dandruff causing organisms such as Malassezia furfur, Trichophyton mentagrophytes, Microsporum gypseum was evaluated using Antifungal Susceptibility Testing-Disk Diffusion Method. Zone of inhibition produced by TRICHONEMTM was high for all the three organisms considered for study. Minimum Inhibitory Concentration (MIC) test revealed that the growth of dandruff causing organisms was reduced due to the effect of TRICHONEMTM hair wash powder. Pilot study considering 50 female volunteers in age range of 22-40 were studied to evaluate the performance of TRICHONEM TM hair wash powder for following parameters such as Dry scalp, Hair fall, Dandruff, Scaling, Pruritus and the results concludes that TRICHONEM TM hair wash powder formulation scored high ratings in treating the hair conditions after 4 weeks of treatment in comparison with placebo. The results from in-vitro anti-dandruff study and pilot study confirm the ability of TRICHONEMTM hair wash powder, which is developed with best ingredients in optimized combination as the best replacement for synthetic counterpart.