Investigating the effect of Polyherbal Ointment on Non-Healing Burn Wound: The Geriatric Approach

Abstract

The most common type of wound is a burn wound which can be complicated due to electrolyte and fluid loss, leads to increase the chance of infectious complications and delay in wound healing. The main objective of the present study is to develop and evaluate a topical polyherbal ointment for antimicrobial and wound healing activity. The polyherbal ointment was formulated by fusion method using emulsifying ointment base with 10%w/w alcoholic extracts (soxhlet extraction) of Azadirachta indica (Neem), Melaleucaalternifolia (tea tree oil), and Myrica esculenta (katphala). The prepared ointment was evaluated for physicochemical property, wound healing and antimicrobial activity. The prepared ointments were tested for its odor, color, consistency, phase separation, homogeneity, pH, spreadability, viscosity, extrudability, and stability studies and gave satisfactory results. The antimicrobial activity of prepared polyherbalointment was evaluated using standard ATCC strain for gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) and gram-negative bacteria (Pseudomonas aeruginosa) using the agar welldiffusion method. The polyherbalointment was investigated in vivo in rats for woundhealing activity. The prepared formulation showed predominant antimicrobial activity against selected species and formulation promoted wound healing activity when compared with marketed (standard povidone ointment) preparation. Overall study reveals that prepared formulation is an effective polyherbal ointment.