Nephroprotective effects of Hydroalcoholic Extracts of Four selected Indian Medicinal Plants in Gentamicin-induced Nephrotoxic Wistar Rats

Abstract

All the selected Indian medicinal plants, Ficus religiosa; Tinospora cordifolia; Moringa oleifera; Boerhaevia diffusa have profound pharmacological uses which have been proved by the study of Ayurvedic literature. An Aminoglycoside like Gentamicin is a broad-spectrum antibiotic in combination with beta-lactams for the treatment of systemic infection. The major risk associated with Gentamicin is nephrotoxicity and ototoxicity The present study was done to evaluate the comparative Nephroprotective effects of four selected Indian medicinal plant extracts in albino Wistar rats in Gentamicin-induced nephrotoxicity. Hydroalcoholic extracts of stem bark of Ficus religiosa, stem of Tinospora cordifolia, leaves of Moringa oleifera and roots of Boerhaevia diffusa were used at two doses levels of 200, and 400mg/kg respectively to evaluate Nephroprotective activity in albino Wistar rats by interfering Body weight, Blood urea nitrogen (BUN), Serum creatinine (Serum Creatinine), Serum total proteins, Urine volume, Urine creatinine, Creatinine clearance with Histopathology of Kidney. To observe the effect of various treatments on animals the rats were divided into Eleven groups, Six in each group. Urine creatinine, serum urea, and blood urea nitrogen were found to be significantly (P < 0.001) increased in rats treated with only gentamicin; whereas treatment with the Hydroalcoholic extract of stem of Tinospora cordifolia and root of Boerhaevia diffusa reversed the effect of gentamicin indicating nephroprotective activity in compared to other plant extract treated animals. The Hydroalcoholic extract of Tinospora cordifolia and Boerhaevia diffusa at a dose level of 400mg/kg was found to be rich in flavonoids, polyphenolics, and other phytoconstituents which are responsible for good nephroprotective action in Gentamicin-induced nephrotoxicity in rats.