Affiliation:
1. Medical Laboratory Sciences Department at Allied Medical Sciences Faculty, Mutah University, AL-KARAK, Jordan.
Abstract
Hydrogen peroxide (H2O2) is reactive oxygen species produced by cellular metabolism in human body. This in vitro study was designed to measure oxidative injury induced in clotting factors and plasma proteins by H2O2 and determined the capability of Gallic acid to protect clotting factors and plasma proteins against H2O2-induced oxidative injury. The capability of Gallic acid to resist H2O2-induced oxidation was estimated by measuring the prothrombin time (PT), activated partial thromboplastin time (aPTT), protein carbonyl and malondialdehyde formation in experimental groups. Incubation of clotting factors and plasma proteins with 0.5% H2O2 for 30 minutes resulted in significantly increase PT, aPTT, and significantly rise levels of malondialdehyde and carbonyl groups formation. Gallic acid significantly decreased PT and aPTT and reduced carbonyl group and malondialdehyde formation in plasma proteins. Therefore, the antioxidant Gallic acid protects clotting factors and plasma proteins from oxidative injury induced by H2O2.
Subject
Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
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