Antimalarial Activity of Nano Phytomedicine Fraction of Syzygium cumini Fruit in Rodent Malaria

Abstract

The purpose of this study is to prove the antimalarial activity of nanophytomedicine fraction Syzygium cumini fruit in rodent malaria. Preparation of nanoparticle formulations using ionic gelation. This research used 80 mice divided into 8 groups. K: not infected, K-: infected, P1: infected+chloroquine, P2: infected+fraction, P3, P4, P5, were infected + nanoparticles, and P6 were infected + nanoparticles combined with chloroquine. Mice were infected with red blood cells containing 1x106 in 0.2ml P.berghei. Treatment for 4 days and 24hours post-infection. On the 8th day, post-infection blood and organs were collected. The results showed the body weight of mice showed a decrease except for the P1 and P6 groups. Increased % parasitemia and decreased parasite growth inhibition in group K- compared to P1, P6, and P5. The splenic index of the K group was significantly different from the other groups. The hepatic index of the K group was not significantly different from the P1 and P6 groups, but significantly different from the other groups. The hematological changes of hemoglobin and hematocrit in groups K- and P3 showed a decrease. Leukocytes, monocytes, and granulocytes in all groups were in the normal range. The conclusion is Nanoparticles fraction of Syzygium cumini at a dose of 400 mg/kg BW and combination therapy with chloroquine have better potential as an antimalarial seen from the decreased parasitemia, increased inhibition of parasite growth, increased body weight, splenic index, hepatic index and Hematological changes of mice infected with Plasmodium berghei.