Comparative evaluation of physicochemical, qualitative and chromatographic profile of unprocessed and processed Cannabis sativa L (Bhanga) leaves-Reference-Cited by-同舟云学术

Comparative evaluation of physicochemical, qualitative and chromatographic profile of unprocessed and processed Cannabis sativa L (Bhanga) leaves

Published:2023-11-30 Issue: Volume: Page:5341-5346
ISSN:0974-360X
Container-title:Research Journal of Pharmacy and Technology
language:en
Short-container-title:RJPT

Author:

Dilip Tavhare Swagata¹,Acharya Rabinarayan²,Vaghora Bhagyashri³,Shukla Vinay⁴

Affiliation:

1. Associate Professor, Department of Dravyaguna, Dr. D. Y. Patil College of Ayurved and Research Center, D.Y. Patil Vidyapeeth (Deemed to be University), Pimpri, Pune, Maharashtra-411018.

2. Director General, Central Council for Research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Government of India, New Delhi - 110058.

3. Pharmacist, Government Ayurved Hospital, Junagadh, Gujarat- 362310.

4. Head, Pharmaceutical Chemistry Laboratory, Institute of Teaching and Research in Ayurveda Jamnagar, Gujarat-361008.

Abstract

Background: Cannabis (Bhanga) has been extensively explored in research for therapeutic benefits. Out of 80+ phyto-cannabinoid detected till date; out of which Tetrahydrocannabinol (THC), Cannabidiol (CBD) and Cannabinol (CBN) are therapeutically important. Psychotropic concern about cannabis is a majorly attributed to THC presence. For reducing intoxicant effect, Ayurveda recommends processing (Shodhana) of cannabis. Objectives: To evaluate impact of cannabis leaves processing techniques through pharmacognosy, photochemistry and chromatography. Material and methods: Four different processing methods; namely water-immersion (Jala-nimajjana) (JN), water-wash (Jala-prakshalana) (JP), boiling in cow’s milk (Godugdha-Swedana) (GS) and boiling in decoction of Acacia arabica (Babbul twak Kwatha-Swedana) (BKS) were performed as mentioned in Ayurveda classics. Water and ethanol extract of respective sample and processing media were prepared. Processed samples were evaluated for physicochemical, qualitative and chromatographic studies (HPTLC) and comparative evaluation was done against markers THC, CBD and CBN. Results: Processed samples showed variation in physico-chemical parameters. Functional groups like carbohydrates, phenols and steroids retained after processing in all samples. Additionally; protein and tannin groups were found in samples processed by GS and BKS method. Processing of cannabis reduced THC, CBD and CBN from samples and which transferred to media as quantified through HPTLC. In GS method, no maker visualized through HPTLC. This may be due to changes in nature of molecules caused by boiling. Reduction of THC found highest in JP (31%) followed by JN (18%) and BKS (16%) method. Conclusion: Processing showed changes in physicochemical parameters of cannabis leaves and reduces its THC content;thus; reducing its intoxicant effect without altering presence of functional group.

Publisher

A and V Publications

Subject

Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Reference23 articles.

1. Sen V. Bhaishajya Ratnavali, Editor, Pandey Gyanendra, Chaukhamba Sanskrit Series office, Varanasi. 2005; Appendix C, First Edition 2005; pp. 529

2. Anonymous. Ministry of Health and Family Welfare (Department of Health), Government of India. Drugs and Cosmetics act 1940 with Drugs and Cosmetics Rules, 2003. Ministry of Health and Family Welfare (Department of Health). Delhi: 1945; pp. 317.

3. Mishra GS. Àyurveda Prakash. Chaukhamba Surabharati Academy, New Delhi, India. 2007; pp. 490–5.

4. Bhavamishra: Bhavprakasha Nighantu. Haritakyadi varga, Commentary by Prof. K. C. Chunekar. Edited by Late Dr. G.S. Pandey; Chaukhamba Bharati Academy, Varanasi.2006; pp.141.

5. Kichloo, A. et al. Marijuana: A systems-based primer of adverse effects associated with use and an overview of its therapeutic utility. SAGE open medicine, 2021; Mar 9; 9:20503121211000909. doi: 10.1177/20503121211000909.