Evaluation of In-vitro Anti-oxidant and α-amylase inhibition activity of Allium tuberosom and its Phytochemical analysis

Abstract

Allium tuberosom, a species native to Chinese province of Shanxi and also found in the North-eastern states of India especially Manipur, is well known for its medicinal properties such as antibacterial, cardiac, stimulant, anti-cancer, etc. Fresh samples of Allium tuberosum were collected from Manipur and assayed for the presence of potent phytochemicals. Moreover, the extracts of the sample were also investigated for their anti-oxidant and anti-diabetic activities. Two solvents of varied polarity were used for soxhlet extraction and standard protocols were employed throughout the study. Presence of various phytochemicals such as alkaloids, flavonoids, saponins, etc. was reported. Upon evaluation of anti-oxidant activity by DPPH method, it was recorded that more the concentration of the sample more was its anti-oxidant activity for both the extracts (hexane and methanol), i.e. upto 63.1% for hexane extract and 67.4% for methanol extract. Anti-diabetic activity by α-amylase inhibition assay was also dependent on its concentration. 64.19% for hexane extract and 62.7% for methanol extract of α-amylase inhibiting activity was recorded. The results of the current study prove the potentiality of the species to be further considered as a valuable asset for clinical trials so as to be explored in pharmaceutical purposes.