Affiliation:
1. Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal - 576104, Udupi, Karnataka.
Abstract
Inulin is a high molecular weight carbohydrate made up of fructose molecules attached with glucosyl moieties in the end. Due to its high molecular weight direct analysis is tedious hence we opted for derivatization. Inulin was derivatized using Seliwanoff’s reagent and was analyzed using UV-spectrophotometry. This method was validated for specificity, linearity, precision, accuracy, the limit of detection (LOD), and the limit of quantitation (LOQ). It was seen that all the parameters met the acceptance criteria. After validation the method was applied for the formulation and %recovery was determined.
Subject
Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Reference20 articles.
1. Shoaib M. Shehzad A. Omar M. Rakha A. Raza H. Sharif HR. et al Inulin: Properties health benefits and food applications. Carbohydrate Polymers 2016;147(444–54):https://doi.org/10.1016/j.carbpol.2016.04.020.
2. USP 29-NF 24 The United States Pharmacopoeia and The National Formulary: The Official Compendia of Standards 29/24. Rockville MD: The United States Pharmacopeial Convention.
3. Barclay T.Ginic-Markovic M. Cooper P.Petrovsky N. Inulin-a versatile polysaccharide with multiple pharmaceutical and food chemical uses. Journal of Excipients and Food Chemicals. 2016;1(3):1132.
4. Abed SM. Ali AH. Noman A.Niazi S. Ammar AF.Bakry AM. Inulin as prebiotics and its applications in food industry and human health; a review. International Journal of Agriculture Innovations and Research. 2016; 5(1): 88-97.
5. Sánchez-Viesca F. Gómez R. Reactivities involved in the Seliwanoff reaction. Modern Chemistry. 2018; 6(1):1-5. https://doi.org/10.11648/j.mc.20180601.11.