Anticancer Activity of Methanol extract of Limnophila repens and Argyeia cymosa by Using SRB Assay

Abstract

Introduction: Plants have a special place in the treatment of cancer. It is estimated that plant-derived compounds constitute more than 50% of anticancer agents. In this present study I attempted an experiment to find out the anti-cancer activity of selected plants Limnophila repens and Argyeia cymosa by using SRB Assay. Material method: I have collected the plants, dried very well and extracted with methanol crude methanol extract of the both plants tested for its anti-cancer activity. Results and disscussionThe anticancer activity of methanolic extracts of Argyreia cymosa and Limnophila repens was determined by sulforhodamine B colorimetric assay. The results of the cytotoxicity of extracts from both plant extracts analysed and The Methanol extract of Limnophila repens extract showed comparable activity to the standard compound, i.e., Adriamycin on Ishikawa and SCC-29B cell lines, respectively. This extract showed TGI, and GI50 was 38.9 and <10 µg/ ml on Ishikawa cell lines and 58.12, <10 and <10 µg/ ml of LC50, TGI and GI50 activity on SCC-29B cell lines respectively. The Methanol extract of Argyeia cymosa showed GI50 was >80 µg/ ml on Ishikawa cell lines and <10 and <10 µg/ ml of, TGI and GI50 activity on SCC-29B cell lines .Estimations based on GI50 values shows that MELR was more active against Ishikawa and SCC-29B cell lines than MEAC on Ishikawa (human endometrial adenocarcinoma) and SCC-29B (human oral cancer) cell lines.