Spectrophotometric method development and validation for determination of pazopanib in the bulk and the formulation

Abstract

The present work is performed on analytical method development with its latter validation study of a simple and accurate UV-Visible spectrophotometric method for the content determination of the Pazopanib HCl (PAZ). The optimum conditions and solvent selection for the analysis of the drug were established. Absorption maxima (λmax) taken for the analysis was 267nm in the purposed medium. Calibration curve was obtained and plotted against the observed values. Validation of the method was performed as per the current ICH guidelines for linearity, accuracy, precision, LOD and LOQ. The method is simple, accurate, sensitive, precise and reproducible. The method can be used in IPQC and QC testing on a commercial stage as well as in small scale labs, as the method is economical and cheap. The analysis is done on UV-Visible spectrophotometer which is easily available in every institute or in small labs as well.